ASGR1 Recombinant antibody, PBS Only (Detector)

ASGR1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240672C4

Cat no : 83691-3-PBS

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Validation Data Gallery

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  WB analysis using 83691-3-RR (same clone as 83691-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00681-3

  Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

• 

  Sample test of MP00681-3

  The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

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  Sample test of MP00681-3

  Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

• 

  IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00681-1

  Cytometric bead array standard curve of MP00681-1, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-2-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00681-3

  Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83691-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

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Product Information

83691-3-PBS targets ASGR1 as part of a matched antibody pair:

MP00681-1: 83691-2-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array)

MP00681-3: 83691-1-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: NM_001671.4
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.