ASGR1 Recombinant antibody, PBS Only (Detector)

ASGR1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240672C4

Cat no : 83691-3-PBS

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Validation Data Gallery

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  WB analysis using 83691-3-RR (same clone as 83691-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00681-3

  Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

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  Sample test of MP00681-3

  The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

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  Sample test of MP00681-3

  Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

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  Cytometric bead array standard curve of MP00681-1

  Cytometric bead array standard curve of MP00681-1, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-2-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00681-3

  Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83691-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

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Product Information

83691-3-PBS targets ASGR1 as part of a matched antibody pair:

MP00681-1: 83691-2-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array)

MP00681-3: 83691-1-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_001671.4
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.