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ASGR1 Recombinant antibody

ASGR1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240672C4

Cat no : 83691-3-RR

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASGR1 Recombinant antibody (83691-3-RR)

    WB analysis using 83691-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ASGR1 Recombinant antibody (83691-3-RR)

    IHC staining of mouse liver using 83691-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ASGR1 Recombinant antibody (83691-3-RR)

    IHC staining of mouse liver using 83691-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83691-3-RR

    Affinity and Kinetic Characterization of 83691-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, human liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83691-3-RR is ASGR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name asialoglycoprotein receptor 1
Calculated Molecular Weight33 kDa
Observed Molecular Weight 42-46 kDa
GenBank Accession NumberNM_001671.4
Gene Symbol ASGR1
Gene ID (NCBI) 432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Protocols

Product Specific Protocols
WB protocol for ASGR1 antibody 83691-3-RRDownload protocol
IHC protocol for ASGR1 antibody 83691-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols