ASF1A Monoclonal antibody, PBS Only

ASF1A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4A6

Cat no : 66884-1-PBS

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

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Validation Data Gallery

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  WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66884-1-Ig (same clone as 66884-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66884-1-Ig (ASF1A antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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Product Information

66884-1-PBS targets ASF1A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASF1A fusion protein Ag1233
• Full Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 204 aa, 23 kDa
• Observed Molecular Weight: 21-23 kDa
• GenBank Accession Number: BC010878
• Gene Symbol: ASF1A
• Gene ID (NCBI): 25842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.