• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ASF1A Monoclonal antibody

ASF1A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4A6

Cat no : 66884-1-Ig

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using ASF1A Monoclonal antibody (66884-1-Ig)

    WB analysis of Jurkat using 66884-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ASF1A Monoclonal antibody (66884-1-Ig)

    WB analysis of HSC-T6 using 66884-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASF1A Monoclonal antibody (66884-1-Ig)

    IF Staining of HepG2 using 66884-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66884-1-Ig (ASF1A antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66884-1-Ig targets ASF1A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 21-23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2882216
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Protocols

Product Specific Protocols
WB protocol for ASF1A antibody 66884-1-IgDownload protocol
IF protocol for ASF1A antibody 66884-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols