ASCL1 Polyclonal antibody, PBS Only

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  WB analysis using 23751-1-AP (same clone as 23751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23751-1-AP (ASCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23751-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 23751-1-AP (same clone as 23751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23751-1-AP (ASCL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23751-1-PBS in a different storage buffer formulation.

ASCL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 23751-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

HASH1, Class A basic helix-loop-helix protein 46, bHLHa46, ASH-1, ASH1

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Product Information

23751-1-PBS targets ASCL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASCL1 fusion protein Ag19360
• Full Name: achaete-scute complex homolog 1 (Drosophila)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC003134
• Gene Symbol: ASCL1
• Gene ID (NCBI): 429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P50553
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.