ASAH1 Monoclonal antibody, PBS Only

• 

  WB analysis using 67092-2-Ig (same clone as 67092-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67092-2-Ig (same clone as 67092-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

ASAH1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67092-2-PBS

Clone No.2B3C7

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

---

Product Information

67092-2-PBS targets ASAH1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

• Tested Reactivity: human, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASAH1 fusion protein Ag28645
• Full Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC016828
• Gene Symbol: ASAH1
• Gene ID (NCBI): 427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13510
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.