ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B3C7

Cat no : 67092-2-Ig

Synonyms

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

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Validation Data Gallery

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  WB analysis using 67092-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 67092-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: pig liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67092-2-Ig targets ASAH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

• Tested Reactivity: human, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASAH1 fusion protein Ag28645
• Full Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC016828
• Gene Symbol: ASAH1
• Gene ID (NCBI): 427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.