ARP3/ARP3B Monoclonal antibody, PBS Only

ARP3/ARP3B Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12C7

Cat no : 68439-1-PBS

Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

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Validation Data Gallery

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  WB analysis using 68439-1-Ig (same clone as 68439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68439-1-Ig (ACTR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARP3/ARP3B antibody (68439-1-Ig, Clone: 1D12C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68439-1-PBS targets ARP3/ARP3B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARP3/ARP3B fusion protein Ag5017
• Full Name: ARP3 actin-related protein 3 homolog (yeast)
• Calculated Molecular Weight: 418 aa 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC044590
• Gene Symbol: ACTR3
• Gene ID (NCBI): 10096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.