ARP3/ARP3B Monoclonal antibody

ARP3/ARP3B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D12C7

Cat no : 68439-1-Ig

Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

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Validation Data Gallery

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  WB analysis using 68439-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68439-1-Ig (ACTR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 68439-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68439-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 68439-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARP3/ARP3B antibody (68439-1-Ig, Clone: 1D12C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Calu-3 cells, A549 cells, U2OS cells, HeLa cells, Human peripheral blood platelets, Pig lung tissue, Rabbit lung tissue, Rat lung tissue, Mouse lung tissue
• Positive IHC detected in: mouse brain tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68439-1-Ig targets ARP3/ARP3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARP3/ARP3B fusion protein Ag5017
• Full Name: ARP3 actin-related protein 3 homolog (yeast)
• Calculated Molecular Weight: 418 aa 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC044590
• Gene Symbol: ARP3/ACTR3
• Gene ID (NCBI): 10096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.