ARNT/HIF-1 beta Monoclonal antibody, PBS Only

ARNT/HIF-1 beta Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66732-1-PBS

Synonyms

ARNT, HIF-1 beta, HIF1B, 3B12C5, HIF1 beta

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Validation Data Gallery

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  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HT-1376 using 66732-1-Ig (same clone as 66732-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-1376 cells using ARNT/HIF-1 beta antibody (66732-1-Ig, Clone: 3B12C5 ) at dilution of 1:1300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66732-1-Ig (same clone as 66732-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT/HIF-1 beta (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

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Product Information

66732-1-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARNT/HIF-1 beta fusion protein Ag5507
• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated Molecular Weight: 416 aa, 46 kDa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC060838
• Gene Symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia