ARNT,HIF1B Monoclonal antibody, PBS Only

ARNT,HIF1B Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66732-1-PBS

Synonyms

ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

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Validation Data Gallery

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  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66732-1-Ig (same clone as 66732-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66732-1-PBS targets ARNT,HIF1B in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARNT,HIF1B fusion protein Ag5507
• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated Molecular Weight: 416 aa, 46 kDa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC060838
• Gene Symbol: ARNT
• Gene ID (NCBI): 405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia