ARNT/HIF-1 beta Recombinant antibody, PBS Only

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  WB analysis using 84650-4-RR (same clone as 84650-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84650-4-RR (ARNT,HIF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARNT,HIF1B antibody (84650-4-RR, Clone: 242111G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84650-4-RR (same clone as 84650-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84650-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84650-4-RR against Human ARNT/HIF-1 beta were performed. The affinity constant is 0.109 nM.

ARNT/HIF-1 beta Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84650-4-PBS

Clone No.242111G2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta

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Product Information

84650-4-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARNT/HIF-1 beta fusion protein Ag5507
• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated Molecular Weight: 416 aa, 46 kDa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC060838
• Gene Symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P27540
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.