ARL4D Recombinant antibody

ARL4D Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241503B7

Cat no : 84163-3-RR

Synonyms

ARF4L, ARL6, ADP-ribosylation factor-like protein 4L, ADP-ribosylation factor-like protein 4D, 241503B7

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Validation Data Gallery

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  WB analysis using 84163-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84163-3-RR (ARL4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84163-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84163-3-RR against Human ARL4D were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Y79 cells, mouse retina tissue, rat retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84163-3-RR is ARL4D Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARL4D fusion protein Ag7369
• Full Name: ADP-ribosylation factor-like 4D
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC000043
• Gene Symbol: ARL4D
• Gene ID (NCBI): 379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane  (PMID: 17804820).