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ARL4D Recombinant antibody, PBS Only

ARL4D Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241503B7

Cat no : 84163-3-PBS

Synonyms

ARF4L, ARL6, ADP-ribosylation factor-like protein 4L, ADP-ribosylation factor-like protein 4D, 241503B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARL4D Recombinant antibody (84163-3-RR)

    WB analysis using 84163-3-RR (same clone as 84163-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84163-3-RR (ARL4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84163-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84163-3-RR

    Affinity and Kinetic Characterization of 84163-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84163-3-RR against Human ARL4D were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 84163-3-PBS is ARL4D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARL4D fusion protein Ag7369
Full Name ADP-ribosylation factor-like 4D
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC000043
Gene Symbol ARL4D
Gene ID (NCBI) 379
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane  (PMID: 17804820).