CoraLite® Plus 647-conjugated ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Canine

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1H6C3

Cat no : CL647-66739

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

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Validation Data Gallery

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  IF Staining of hTERT-RPE1 using CL647-66739

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 647 ARL13B antibody (CL647-66739, Clone: 1H6C3 ) at dilution of 1:200.

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  IF Staining of MDCK using CL647-66739

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 647-conjugated ARL13B antibody (CL647-66739, Clone: 1H6C3 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66739 targets ARL13B in IF applications and shows reactivity with Human, Canine samples.

• Tested Reactivity: Human, Canine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12031
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• RRID: AB_2920288
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).