ARL13B Recombinant antibody, PBS Only

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  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82834-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82834-2-RR against Human ARL13B were performed. The affinity constant is 1.25 nM.

ARL13B Uni-rAb^TM Recombinant Antibody for IF/ICC, Indirect ELISA

Cat No. 82834-2-PBS

Clone No.3H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

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Product Information

82834-2-PBS targets ARL13B in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q3SXY8
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.