ARL13B Monoclonal antibody, PBS Only

ARL13B Monoclonal Antibody for WB, IF/ICC, IF-Fro, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, canine

Applications

WB, IF/ICC, IF-Fro, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H6C3

Cat no : 66739-1-PBS

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

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Validation Data Gallery

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  WB analysis of HepG2 using 66739-1-Ig (same clone as 66739-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66739-1-Ig (same clone as 66739-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66739-1-Ig (same clone as 66739-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66739-1-Ig (same clone as 66739-1-PBS)

  rozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T /cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66739-1-Ig (same clone as 66739-1-PBS)

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 66739-1-Ig (same clone as 66739-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL13B antibody (66739-1-Ig, Clone: 1H6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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Product Information

66739-1-PBS targets ARL13B in WB, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, pig, canine samples.

• Tested Reactivity: human, mouse, pig, canine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12031
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).