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Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugate

Unconjugated

Cat no : 15513-1-AP

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    WB analysis of mouse heart using 15513-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    WB analysis of rat heart using 15513-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    WB analysis of mouse skeletal muscle using 15513-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    WB analysis of human heart using 15513-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (TNNT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IHC staining of mouse heart using 15513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IHC staining of mouse heart using 15513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IHC staining of mouse skeletal muscle using 15513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IHC staining of mouse skeletal muscle using 15513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IF Staining of mouse heart using 15513-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin T antibody (15513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IF Staining of mouse heart using 15513-1-AP

    Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Cardiac Troponin T Polyclonal antibody (15513-1-AP)

    IF Staining of mouse heart using 15513-1-AP

    Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF-Fro detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15513-1-AP targets Cardiac Troponin T in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag7716
Full Name troponin T type 2 (cardiac)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC002653
Gene Symbol Cardiac Troponin T
Gene ID (NCBI) 7139
RRIDAB_2206563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
IHC protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
IF protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Brahma safeguards canalization of cardiac mesoderm differentiation.

Authors - Swetansu K Hota
View Article
pigIF

Cell Stem Cell

Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation

Authors - Jiaowei Wang
View Article
humanIF

Sci Transl Med

Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy

Authors - Takahiko Nishiyama
View Article
humanIF

ACS Nano

Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Authors - Yiqi Gong
View Article
mouseIF

Acta Pharm Sin B

Hippo pathway-manipulating neutrophil-mimic hybrid nanoparticles for cardiac ischemic injury via modulation of local immunity and cardiac regeneration

Authors - Qiaozi Wang
View Article
mouseIHC

Dev Cell

A Multifunctional Protein, EWS, Is Essential for Early Brown Fat Lineage Determination.

Authors - Park Jun Hong JH
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Rebecca (Verified Customer) (03-16-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: iPS-CM
Cardiac Troponin T Antibody Western Blot validation (1:1000 dilution) in iPS-CM (Cat no:15513-1-AP)
FH

Lianjie (Verified Customer) (07-26-2019)

This antibody works well.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse embryonic heart
Cardiac Troponin T Antibody Immunohistochemistry, validation (1:50 dilution) in Mouse embryonic heart (Cat no:15513-1-AP)