ARID4A Recombinant antibody, PBS Only (Capture)

ARID4A Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240436A4

Cat no : 83489-1-PBS

Synonyms

RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A

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Validation Data Gallery

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  WB analysis using 83489-1-RR (same clone as 83489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83489-1-RR (ARID4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00489-3

  Sandwich ELISA standard curve of MP00489-3, Human ARID4A Recombinant Matched Antibody Pair - PBS only. 83489-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33114. 83489-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00489-3

  The mean ARID4A concentration was determined to be 9.3 ng/mL in DU 145 cell extract based on a 3.2 mg/mL extract load, 18.1 ng/mL in MCF-7 cell extract based on a 3.5 mg/mL extract load.

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  IHC staining of mouse brain using 83489-1-RR (same clone as 83489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83489-1-RR (ARID4A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83489-1-RR (same clone as 83489-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ARID4A antibody (83489-1-RR, Clone: 240436A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83489-1-RR (same clone as 83489-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ARID4A Recombinant antibody (83489-1-RR, Clone:240436A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00489-1

  Cytometric bead array standard curve of MP00489-1, ARID4A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83489-1-PBS. Detection antibody: 83489-4-PBS. Standard: Ag33114. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83489-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83489-1-RR against Human ARID4A were performed. The affinity constant is below 1 pM.

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Product Information

83489-1-PBS targets ARID4A as part of a matched antibody pair:

MP00489-1: 83489-1-PBS capture and 83489-4-PBS detection (validated in Cytometric bead array)

MP00489-3: 83489-1-PBS capture and 83489-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARID4A fusion protein Ag33114
• Full Name: AT rich interactive domain 4A (RBP1-like)
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 205 kDa
• GenBank Accession Number: NM_002892.3
• Gene Symbol: ARID4A
• Gene ID (NCBI): 5926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.