CoraLite® Plus 488-conjugated Arginase-1 Polyclonal antibody

Arginase-1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16001

Synonyms

ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-16001

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ARG1 antibody (CL488-16001) at dilution of 1:400.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16001 targets Arginase-1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Arginase-1 fusion protein Ag8595
• Full Name: arginase, liver
• Calculated Molecular Weight: 236aa,25 kDa; 322aa,35 kDa
• Observed Molecular Weight: 35-36 kDa
• GenBank Accession Number: BC005321
• Gene Symbol: Arginase-1
• Gene ID (NCBI): 383
• RRID: AB_2919127
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protect motor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539). It can exist as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing. Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 has 3 isoforms with the molecular mass of 25, 35 and 36 kDa.