ARF6 Monoclonal antibody

ARF6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5H1

Cat no : 68924-1-Ig

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1

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Validation Data Gallery

• 

  WB analysis of LNCaP using 68924-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 68924-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68924-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 68924-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, MCF-7 cells, pig liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68924-1-Ig targets ARF6 in WB, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARF6 fusion protein Ag12642
• Full Name: ADP-ribosylation factor 6
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC002952
• Gene Symbol: ARF6
• Gene ID (NCBI): 382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.