ARF1 Monoclonal antibody, PBS Only

ARF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10E3

Cat no : 68069-1-PBS

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

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Validation Data Gallery

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  WB analysis using 68069-1-Ig (same clone as 68069-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68069-1-Ig (ARF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

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Product Information

68069-1-PBS targets ARF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

• Tested Reactivity: rabbit, pig, rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARF1 fusion protein Ag28021
• Full Name: ADP-ribosylation factor 1
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC009247
• Gene Symbol: ARF1
• Gene ID (NCBI): 375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).