ARF1 Monoclonal antibody

ARF1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C10E3

Cat no : 68069-1-Ig

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

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Validation Data Gallery

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  WB analysis using 68069-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68069-1-Ig (ARF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 68069-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 68069-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 68069-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

68069-1-Ig targets ARF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARF1 fusion protein Ag28021
• Full Name: ADP-ribosylation factor 1
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC009247
• Gene Symbol: ARF1
• Gene ID (NCBI): 375
• RRID: AB_2918810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).

Publications

Species	Application	Title
human	WB,IP	Cell Metab; Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation; Authors - Jian Zhou