CoraLite®594-conjugated ARC Monoclonal antibody

• Featured Product
• KD/KO Validated

ARC Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G4B5

Cat no : CL594-66550

Synonyms

ARC, ARC/ARG3.1, Arg3.1, KIAA0278

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Validation Data Gallery

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  IF Staining of SH-SY5Y using CL594-66550

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®594 ARC antibody (CL594-66550, Clone: 1G4B5 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66550 targets ARC in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARC fusion protein Ag9445
• Full Name: activity-regulated cytoskeleton-associated protein
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC012321
• Gene Symbol: ARC
• Gene ID (NCBI): 23237
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.