CoraLite® Plus 488-conjugated AQP1 Monoclonal antibody

AQP1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E5D6

Cat no : CL488-66805

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

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Validation Data Gallery

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  IF Staining of human kidney using CL488-66805

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-66805, Clone: 2E5D6 ) at dilution of 1:200.

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  IF Staining of human kidney using CL488-66805

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-66805, Clone: 2E5D6 ) at dilution of 1:400, CoraLite®594 Podocalyxin antibody (CL594-18150, red).

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Tested Applications

• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66805 targets AQP1 in IF-P applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AQP1 fusion protein Ag14093
• Full Name: aquaporin 1 (Colton blood group)
• Calculated Molecular Weight: 269 aa, 29 kDa
• Observed Molecular Weight: 28 kDa, 38-40 kDa
• GenBank Accession Number: BC022486
• Gene Symbol: AQP1
• Gene ID (NCBI): 358
• RRID: AB_2919375
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)