AQP1 Monoclonal antibody, PBS Only

AQP1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5D6

Cat no : 66805-1-PBS

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

---

Validation Data Gallery

• 

  WB analysis of human heart using 66805-1-Ig (same clone as 66805-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 66805-1-Ig (same clone as 66805-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody (66805-1-Ig, Clone: 2E5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66805-1-PBS targets AQP1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AQP1 fusion protein Ag14093
• Full Name: aquaporin 1 (Colton blood group)
• Calculated Molecular Weight: 269 aa, 29 kDa
• Observed Molecular Weight: 28 kDa, 38-40 kDa
• GenBank Accession Number: BC022486
• Gene Symbol: AQP1
• Gene ID (NCBI): 358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)