APPL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

APPL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 19885-1-AP

Synonyms

DIP13alpha, DIP13A, Dip13 alpha, DCC-interacting protein 13-alpha, APPL

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Validation Data Gallery

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  WB analysis of human brain using 19885-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19885-1-AP

  WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 19885-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19885-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 19885-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19885-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 19885-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19885-1-AP

  IP result of anti-APPL1 (IP:19885-1-AP, 4ug; Detection:19885-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human breast cancer using 19885-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 19885-1-AP (APPL1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 19885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 19885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 19885-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, HeLa cells, human heart tissue, HT-1080 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19885-1-AP targets APPL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APPL1 fusion protein Ag13703
• Full Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Calculated Molecular Weight: 709 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC028599
• Gene Symbol: APPL1
• Gene ID (NCBI): 26060
• RRID: AB_10637848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].