human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 19885-1-AP
WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.
WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.
WB analysis of HeLa using 19885-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 19885-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19885-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 19885-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 19885-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HT-1080 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 19885-1-AP
IP result of anti-APPL1 (IP:19885-1-AP, 4ug; Detection:19885-1-AP 1:1000) with mouse brain tissue lysate 2640ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 19885-1-AP (APPL1 Antibody) at dilution of 1:50.
IF Staining of HepG2 using 19885-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 19885-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 19885-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue, HEK-293 cells, HeLa cells, human heart tissue, HT-1080 cells, mouse brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
19885-1-AP targets APPL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 19885-1-AP
WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.
WB analysis of HeLa using 19885-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 19885-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19885-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 19885-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 19885-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 19885-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 19885-1-AP (APPL1 Antibody) at dilution of 1:50.
IP Figures
IP experiment of mouse brain using 19885-1-AP
IP result of anti-APPL1 (IP:19885-1-AP, 4ug; Detection:19885-1-AP 1:1000) with mouse brain tissue lysate 2640ug.
IF/ICC Figures
IF Staining of HepG2 using 19885-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 19885-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using 19885-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
APPL1 Polyclonal antibody
Catalog Number
19885-1-AP
Citations
-
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
