APP Monoclonal antibody, PBS Only

APP Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C2A1

Cat no : 60342-1-PBS

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta

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Validation Data Gallery

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  WB analysis of Y79 using 60342-1-Ig (same clone as 60342-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60342-1-Ig (same clone as 60342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60342-1-Ig (same clone as 60342-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60342-1-PBS targets APP in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat, rabbit samples.

• Tested Reactivity: human, pig, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: APP fusion protein Ag0769
• Full Name: amyloid beta (A4) precursor protein
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC004369
• Gene Symbol: APP
• Gene ID (NCBI): 351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).