APOL1-Specific Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

APOL1-Specific Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12D11

Cat no : 66124-1-PBS

Synonyms

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

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Validation Data Gallery

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  WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

  1.5 ug human plasma was subjected to SDS PAGE followed by western blot with 66124-1-Ig (APOL1 Antibody) at dilution of 1:400, 000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

  IP result of anti-APOL1-Specific (IP:66124-1-Ig, 5ug; Detection:66124-1-Ig 1:10000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

  1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (66124-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66124-1-PBS targets APOL1-Specific in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOL1-Specific fusion protein Ag2016
• Full Name: apolipoprotein L, 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC017331
• Gene Symbol: APOL1
• Gene ID (NCBI): 8542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.