HRP-conjugated APOB Polyclonal antibody

APOB Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

HRP

Cat no : HRP-20578

Synonyms

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB

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Validation Data Gallery

• 

  WB analysis of mouse liver using HRP-20578

  mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using HRP-20578

  human plasma were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using HRP-20578

  mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-20578 targets APOB in WB applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: apolipoprotein B (including Ag(x) antigen)
• Calculated Molecular Weight: 516 kDa
• Observed Molecular Weight: 150-250 kDa, 400-520 kDa
• GenBank Accession Number: NM_000384
• Gene Symbol: APOB
• Gene ID (NCBI): 338
• Conjugate: HRP
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).