CoraLite®594-conjugated APBB1 Monoclonal antibody

APBB1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B9G10

Cat no : CL594-67077

Synonyms

APBB1, FE65, Protein Fe65, RIR

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-67077

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 APBB1 antibody (CL594-67077, Clone: 1B9G10 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-67077

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 APBB1 antibody (CL594-67077, Clone: 1B9G10 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67077 targets APBB1 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: APBB1 fusion protein Ag27436
• Full Name: amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
• Calculated Molecular Weight: 708 aa, 77 kDa
• GenBank Accession Number: BC010854
• Gene Symbol: APBB1
• Gene ID (NCBI): 322
• RRID: AB_2920084
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).