AP4B1 Polyclonal antibody, PBS Only

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  WB analysis using 16313-1-AP (same clone as 16313-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16313-1-AP (AP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16313-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16313-1-AP (same clone as 16313-1-PBS)

  IP result of anti-AP4B1 (IP:16313-1-AP, 4ug; Detection:16313-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16313-1-PBS in a different storage buffer formulation.

AP4B1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 16313-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

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Product Information

16313-1-PBS targets AP4B1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP4B1 fusion protein Ag9348
• Full Name: adaptor-related protein complex 4, beta 1 subunit
• Calculated Molecular Weight: 739 aa, 83 kDa
• Observed Molecular Weight: 75-83 kDa
• GenBank Accession Number: BC014146
• Gene Symbol: AP4B1
• Gene ID (NCBI): 10717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6B7
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.