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  • Western Blot (WB) analysis of various lysates using AP4B1 Polyclonal antibody (16313-1-AP)

    WB analysis using 16313-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16313-1-AP (AP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AP4B1 Polyclonal antibody (16313-1-AP)

    IP experiment of HeLa using 16313-1-AP

    IP result of anti-AP4B1 (IP:16313-1-AP, 4ug; Detection:16313-1-AP 1:300) with HeLa cells lysate 3200ug.

AP4B1 Polyclonal antibody

AP4B1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16313-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, MDA-MB-231 cells, MDA-MB-453s cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16313-1-AP targets AP4B1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP4B1 fusion protein Ag9348 Predict reactive species
Full Name adaptor-related protein complex 4, beta 1 subunit
Calculated Molecular Weight 739 aa, 83 kDa
Observed Molecular Weight75-83 kDa
GenBank Accession NumberBC014146
Gene Symbol AP4B1
Gene ID (NCBI) 10717
RRIDAB_3085485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6B7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.

Protocols

Product Specific Protocols
WB protocol for AP4B1 antibody 16313-1-APDownload protocol
IP protocol for AP4B1 antibody 16313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols