AP1M1 Recombinant antibody, PBS Only

AP1M1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240553H3

Cat no : 83613-6-PBS

Synonyms

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

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Validation Data Gallery

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  WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83613-6-RR (same clone as 83613-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83613-6-RR (same clone as 83613-6-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83613-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83613-6-RR against Human AP1M1 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83613-6-PBS is AP1M1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AP1M1 fusion protein Ag2757
• Full Name: adaptor-related protein complex 1, mu 1 subunit
• Calculated Molecular Weight: 423 aa, 49 kDa
• GenBank Accession Number: BC017469
• Gene Symbol: AP1M1
• Gene ID (NCBI): 8907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.