Annexin A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

36

Reviews (1)

Cat no : 21990-1-AP

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

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Validation Data Gallery

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  WB analysis using 21990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21990-1-AP

  WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.

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  WB analysis of multi-cells using 21990-1-AP

  WB result of 21990-1-AP (Annexin A1 antibody).

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  WB analysis of C6 using 21990-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21990-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 21990-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 21990-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21990-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21990-1-AP

  IP result of anti-Annexin A1 (IP:21990-1-AP, 4ug; Detection:21990-1-AP 1:20000) with HeLa cells lysate 1120 ug.

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  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 21990-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of NIH/3T3 using 21990-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, multi-cells, MDA-MB-453s cells, HeLa cells, U-937 cells, HepG2 cells, MCF-7 cells, C6 cells, A549 cells, K-562 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon tissue, human kidney tissue, human lung tissue, human oesophagus tissue, human ovary tumor tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 10 publications below
• IP: See 4 publications below

Product Information

21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A1 fusion protein Ag17273
• Full Name: annexin A1
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001275
• Gene Symbol: Annexin A1
• Gene ID (NCBI): 301
• RRID: AB_11182596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Publications

Species	Application	Title
mouse	WB,IF	Nature; Evolutionarily divergent mTOR remodels translatome for tissue regeneration; Authors - Olena Zhulyn
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
human	WB	Oncogene; The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis; Authors - Yapeng Wang ; KD Validated
human	WB,IHC,IF,IP	Elife; ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.; Authors - Horia Vais
human	WB	Mol Ther; Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.; Authors - Sen Chandra Sreetama
human	WB	Cancer Immunol Immunother; Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.; Authors - Xiuxing Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (05-23-2022)

excellent with 293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

Annexin A1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:21990-1-AP)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C