ANTXR1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ANTXR1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15091-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 15091-1-AP (same clone as 15091-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15091-1-AP (same clone as 15091-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-13 using 15091-1-AP (same clone as 15091-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 15091-1-AP (same clone as 15091-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15091-1-AP (same clone as 15091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15091-1-AP (same clone as 15091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15091-1-AP (same clone as 15091-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

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Product Information

15091-1-PBS targets ANTXR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANTXR1 fusion protein Ag4626
• Full Name: anthrax toxin receptor 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 85 kDa, 80 kDa
• GenBank Accession Number: BC012074
• Gene Symbol: ANTXR1
• Gene ID (NCBI): 84168
• RRID: AB_2056891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).