ANTXR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ANTXR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15091-1-AP

Synonyms

TEM8, Tumor endothelial marker 8, ATR, Anthrax toxin receptor 1

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Validation Data Gallery

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  WB analysis of mouse testis using 15091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15091-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 15091-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15091-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 15091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 15091-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells, mouse lung tissue, PC-13 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15091-1-AP targets ANTXR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANTXR1 fusion protein Ag4626
• Full Name: anthrax toxin receptor 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 85 kDa, 80 kDa
• GenBank Accession Number: BC012074
• Gene Symbol: ANTXR1
• Gene ID (NCBI): 84168
• RRID: AB_2056891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).

Publications

Species	Application	Title
human	WB,IHC	Am J Transl Res; MicroRNA-26b-3p/ANTXR1 signaling modulates proliferation, migration, and apoptosis of glioma.; Authors - Fei Geng
human	WB	Cancer Manag Res; KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1.; Authors - Yuanchun Li ; KD Validated
human	WB	Comput Math Methods Med; miR-381-3p Involves in Glioma Progression by Suppressing Tumor-Promoter Factor ANTXR1.; Authors - Zhiqiang Dong
mouse	WB,IF	J Cell Physiol; Mechanical strain regulates osteogenesis via Antxr1/LncRNA H19/Wnt/β-catenin axis; Authors - Lifei Liu