CD13 Monoclonal antibody

CD13 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

2D8D11

Cat no : 66211-1-Ig

Synonyms

AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M, Alanyl aminopeptidase

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Validation Data Gallery

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  WB analysis of U-937 using 66211-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66211-1-Ig (CD13 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 66211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD13 antibody (66211-1-Ig, Clone: 2D8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 66211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66211-1-Ig (CD13 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of THP-1 using 66211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (66211-1-Ig, Clone: 2D8D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IHC detected in: human tonsillitis tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue, human kidney tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:8000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66211-1-Ig targets CD13 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD13 fusion protein Ag5976
• Full Name: alanyl (membrane) aminopeptidase
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC058928
• Gene Symbol: CD13
• Gene ID (NCBI): 290
• RRID: AB_2881602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M-1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Profiling the Surfaceome Identifies Therapeutic Targets for Cells with Hyperactive mTORC1 Signaling.; Authors - Junnian Wei
human	WB	Sci Rep; Proteomic Profiling of Small Extracellular Vesicles Secreted by Human Pancreatic Cancer Cells Implicated in Cellular Transformation.; Authors - Kelly A Servage
human	IF	Cancer Manag Res; Liver-enriched activator protein 1 as an isoform of CCAAT/enhancer-binding protein beta suppresses stem cell features of hepatocellular carcinoma.; Authors - Li-Hua Yang
		Biol Pharm Bull; Spautin-A41 Attenuates Cerulein-Induced Acute Pancreatitis through Inhibition of Dysregulated Autophagy.; Authors - Kai Dong
human	WB	Transl Cancer Res; Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer; Authors - Yunyan Shi
mouse	IF	bioRxiv; Suppressing mortality and curbing cellular damage by targeting VHL; Authors - Wei I Jiang