CD13 Monoclonal antibody, PBS Only (Capture)

CD13 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8D11

Cat no : 66211-1-PBS

Synonyms

AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M, Alanyl aminopeptidase

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Validation Data Gallery

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  WB analysis of U-937 using 66211-1-Ig (same clone as 66211-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66211-1-Ig (CD13 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66211-1-Ig (CD13 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD13 antibody (66211-1-Ig, Clone: 2D8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66211-1-Ig (CD13 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 66211-1-Ig (same clone as 66211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (66211-1-Ig, Clone: 2D8D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66211-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50521-1

  Cytometric bead array standard curve of MP50521-1, ANPEP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66211-1-PBS. Detection antibody: 66211-2-PBS. Standard:Ag5976. Range: 0.781-100 ng/mL.

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Product Information

66211-1-PBS targets CD13 as part of a matched antibody pair:

MP50521-1: 66211-1-PBS capture and 66211-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD13 fusion protein Ag5976
• Full Name: alanyl (membrane) aminopeptidase
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC058928
• Gene Symbol: CD13
• Gene ID (NCBI): 290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M-1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)