CoraLite® Plus 488-conjugated ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5A1F12

Cat no : CL488-66160

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66160

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ANP32B antibody (CL488-66160, Clone: 5A1F12 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66160 targets ANP32B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANP32B fusion protein Ag1274
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC013003
• Gene Symbol: ANP32B
• Gene ID (NCBI): 10541
• RRID: AB_2919292
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).