ANAPC10 Recombinant antibody

ANAPC10 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240492E7

Cat no : 83570-4-RR

Synonyms

ANAPC10, APC10, Cyclosome subunit 10, DKFZp564L0562, DOC1

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Validation Data Gallery

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  IF Staining of HeLa using 83570-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANAPC10 antibody (83570-4-RR, Clone: 240492E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83570-4-RR is ANAPC10 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ANAPC10 fusion protein Ag8487
• Full Name: anaphase promoting complex subunit 10
• Calculated Molecular Weight: 185 aa, 21 kDa
• GenBank Accession Number: BC005217
• Gene Symbol: ANAPC10
• Gene ID (NCBI): 10393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The anaphase promoting complex/cyclosome (APC/C) is a cell cycle-regulated multimeric E3 ubiquitin ligase assembled from 13 individual subunits. The anaphase promoting complex subunit 10 (APC10) is a core subunit of APC/C that is highly conserved in humans. APC10 genetically and physically interacts with a series of subunits of the APC/C and is necessary for the ubiquitination activity of APC/C by enhancing the affinity of the APC/C for its substrate.