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- KD/KO Validated
AMPK Alpha 1 Monoclonal antibody
AMPK Alpha 1 Monoclonal Antibody for IHC, WB, ELISA
Cat no : 66536-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, A549 cells, HepG2 cells, K-562 cells, PC-12 cells, L02 cells, HT-29 cells, THP-1 cells, 4T1 cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66536-1-Ig targets AMPK Alpha 1 in WB, IHC, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
|Tested Reactivity||Human, Rat, Mouse|
|Cited Reactivity||human, mouse, rat, goat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||AMPK Alpha 1 fusion protein Ag24744|
|Full Name||protein kinase, AMP-activated, alpha 1 catalytic subunit|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||64 kDa|
|GenBank accession number||BC012622|
|Gene ID (NCBI)||5562|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.
Stem Cells Int
Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway.
Cell Death Dis
WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.
J Immunol Res
Metformin Improves the Prognosis of Adult Mice with Sepsis-Associated Encephalopathy Better than That of Aged Mice.
Int J Mol Sci
Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.
Exp Ther Med
Paeoniflorin suppresses allergic and inflammatory responses by promoting autophagy in rats with urticaria.