AMOTL2 Monoclonal antibody, PBS Only

AMOTL2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C4A4

Cat no : 68401-1-PBS

Synonyms

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

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Validation Data Gallery

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  WB analysis using 68401-1-Ig (same clone as 68401-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 68401-1-Ig (same clone as 68401-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

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  WB analysis of Saos-2 using 68401-1-Ig (same clone as 68401-1-PBS)

  Saos-2 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 68401-1-Ig (same clone as 68401-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68401-1-Ig (same clone as 68401-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AMOTL2 antibody (68401-1-Ig, Clone: 2C4A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68401-1-PBS targets AMOTL2 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AMOTL2 fusion protein Ag19971
• Full Name: angiomotin like 2
• Calculated Molecular Weight: 779 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC011454
• Gene Symbol: AMOTL2
• Gene ID (NCBI): 51421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)