CoraLite® Plus 488-conjugated AMOT Polyclonal antibody

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  IF Staining of HEK-293 using CL488-24550

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 AMOT antibody (CL488-24550) at dilution of 1:200.

• Featured Product
• KD/KO Validated

AMOT Polyclonal Antibody for IF/ICC

Cat No. CL488-24550

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

KIAA1071, Angiomotin

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24550 targets AMOT in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMOT fusion protein Ag19784
• Full Name: angiomotin
• Calculated Molecular Weight: 1084 aa, 118 kDa
• Observed Molecular Weight: 80 kDa, 130 kDa
• GenBank Accession Number: BC130294
• Gene Symbol: AMOT
• Gene ID (NCBI): 154796
• RRID: AB_3672768
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q4VCS5
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin.This antibody recognizes both p130 and p80.