AMIGO3 Polyclonal antibody, PBS Only

AMIGO3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21278-1-PBS

Synonyms

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 21278-1-AP (same clone as 21278-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 21278-1-AP (same clone as 21278-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 21278-1-AP (same clone as 21278-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21278-1-AP (same clone as 21278-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 21278-1-AP (same clone as 21278-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 21278-1-AP (same clone as 21278-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 21278-1-AP (same clone as 21278-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21278-1-AP (AMIGO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21278-1-PBS in a different storage buffer formulation.

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Product Information

21278-1-PBS targets AMIGO3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMIGO3 fusion protein Ag15790
• Full Name: adhesion molecule with Ig-like domain 3
• Calculated Molecular Weight: 504 aa, 55 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC110418
• Gene Symbol: AMIGO3
• Gene ID (NCBI): 386724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.