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AMIGO3 Polyclonal antibody

AMIGO3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21278-1-AP

Synonyms

Amphoterin induced protein 3, AMIGO-3, AMIGO 3, Alivin-3, Alivin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AMIGO3 Polyclonal antibody (21278-1-AP)

    WB analysis of MCF-7 using 21278-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AMIGO3 Polyclonal antibody (21278-1-AP)

    WB analysis of HeLa using 21278-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using AMIGO3 Polyclonal antibody (21278-1-AP)

    WB analysis of human brain using 21278-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using AMIGO3 Polyclonal antibody (21278-1-AP)

    WB analysis of mouse ovary using 21278-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AMIGO3 Polyclonal antibody (21278-1-AP)

    WB analysis of mouse kidney using 21278-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AMIGO3 Polyclonal antibody (21278-1-AP)

    WB analysis of L02 using 21278-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AMIGO3 Polyclonal antibody (21278-1-AP)

    IF Staining of MCF-7 using 21278-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21278-1-AP (AMIGO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, human brain tissue, L02 cells, mouse kidney tissue, mouse ovary tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21278-1-AP targets AMIGO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMIGO3 fusion protein Ag15790
Full Name adhesion molecule with Ig-like domain 3
Calculated Molecular Weight 504 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC110418
Gene Symbol AMIGO3
Gene ID (NCBI) 386724
RRIDAB_10733101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AMIGO3 antibody 21278-1-APDownload protocol
IF protocol for AMIGO3 antibody 21278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols