- Featured Product
- KD/KO Validated
AMH Polyclonal antibody
AMH Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 14461-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | mouse ovary tissue, human pancreas tissue, rat ovary tissue, human ovary tumor tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse ovary tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
14461-1-AP targets AMH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AMH fusion protein Ag5670 |
| Full Name | anti-Mullerian hormone |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC049194 |
| Gene Symbol | AMH |
| Gene ID (NCBI) | 268 |
| RRID | AB_10915986 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa. This antibody is specific to AMH.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMH antibody 14461-1-AP | Download protocol |
| IHC protocol for AMH antibody 14461-1-AP | Download protocol |
| IF protocol for AMH antibody 14461-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theriogenology miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens
| ||
Tissue Cell HGF-modified human umbilical cord mesenchymal stem cells rescue impaired ovarian reserve function in chemotherapy-induced POI rats by improving angiogenesis while decreasing apoptosis and fibrosis in the ovary | ||
Theriogenology Thyroxine regulates pig Sertoli cell line proliferation and maturation through the IKK/NFκB and p38 MAPK signaling pathways | ||
Hum Reprod Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis. |
