AMH Polyclonal antibody

• Featured Product
• KD/KO Validated

AMH Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14461-1-AP

Synonyms

Muellerian-inhibiting factor, Muellerian inhibiting factor, Anti-Muellerian hormone (AMH), Anti-Muellerian hormone, anti Mullerian hormone

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Validation Data Gallery

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  WB analysis of human testis using 14461-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14461-1-AP (AMH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: mouse ovary tissue, human pancreas tissue, rat ovary tissue, human ovary tumor tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

14461-1-AP targets AMH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMH fusion protein Ag5670
• Full Name: anti-Mullerian hormone
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC049194
• Gene Symbol: AMH
• Gene ID (NCBI): 268
• RRID: AB_10915986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa. This antibody is specific to AMH.

Publications

Species	Application	Title
mouse	WB	Reprod Sci; Menstrual Blood-Derived Endometrial Stem Cells Ameliorate Ovarian Senescence by Relieving Oxidative Stress-Induced Inflammation; Authors - Haofeng Song
chicken	WB	Theriogenology; miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens; Authors - Bei-Bei Zhang ; KD Validated
rat	WB	Comb Chem High Throughput Screen; Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve; Authors - Wanjing Li
mouse	IHC	Gut Microbes; High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis; Authors - Jingyi Wen
goat	IF	J Anim Sci Biotechnol; Single-cell transcriptomic atlas of goat ovarian aging; Authors - Dejun Xu
human	IHC	Tissue Cell; HGF-modified human umbilical cord mesenchymal stem cells rescue impaired ovarian reserve function in chemotherapy-induced POI rats by improving angiogenesis while decreasing apoptosis and fibrosis in the ovary; Authors - Junyu Chen