ALR Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ALR Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11293-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11293-1-AP (same clone as 11293-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11293-1-AP (same clone as 11293-1-PBS)

  WB result of ALR antibody (11293-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALR transfected HepG2 cells. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11293-1-AP (same clone as 11293-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 11293-1-AP (same clone as 11293-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 11293-1-AP (same clone as 11293-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 11293-1-AP (same clone as 11293-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 11293-1-AP (same clone as 11293-1-PBS)

  IP result of anti-ALR (IP:11293-1-AP, 3ug; Detection:11293-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11293-1-AP (ALR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11293-1-AP (ALR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11293-1-AP (ALR antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 11293-1-AP (same clone as 11293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 11293-1-AP (same clone as 11293-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ALR antibody (11293-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 11293-1-AP (same clone as 11293-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ALR antibody (11293-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab. This data was developed using the same antibody clone with 11293-1-PBS in a different storage buffer formulation.

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Product Information

11293-1-PBS targets ALR in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALR fusion protein Ag1840
• Full Name: growth factor, augmenter of liver regeneration
• Calculated Molecular Weight: 15 kDa, 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC028348
• Gene Symbol: GFER
• Gene ID (NCBI): 2671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).