ALR Polyclonal antibody

• Featured Product
• KD/KO Validated

ALR Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 11293-1-AP

Synonyms

GFER, HERV1, Hepatopoietin, FAD-linked sulfhydryl oxidase ALR, ERV1

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Validation Data Gallery

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  WB analysis using 11293-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11293-1-AP

  WB result of ALR antibody (11293-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALR transfected HepG2 cells.

• 

  WB analysis of mouse liver using 11293-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11293-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11293-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11293-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11293-1-AP

  IP result of anti-ALR (IP:11293-1-AP, 3ug; Detection:11293-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11293-1-AP (ALR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11293-1-AP (ALR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11293-1-AP (ALR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11293-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11293-1-AP (ALR antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse kidney using 11293-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ALR antibody (11293-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab.

• 

  IF Staining of mouse kidney using 11293-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ALR antibody (11293-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab.

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Tested Applications

• Positive WB detected in: mouse liver tissue, A431 cells, A375 cells, rat liver tissue, HepG2 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue, human testis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

11293-1-AP targets ALR in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALR fusion protein Ag1840
• Full Name: growth factor, augmenter of liver regeneration
• Calculated Molecular Weight: 15 kDa, 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC028348
• Gene Symbol: GFER
• Gene ID (NCBI): 2671
• RRID: AB_2109970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh ; KD Validated
human,mouse	WB	Hepatology; Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation.; Authors - Chao Zhang ; KD Validated
human	WB,IF	Am J Hum Genet; The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency.; Authors - Di Fonzo Alessio A
human	WB,IF	Aging Cell; Dependence of PINK1 accumulation on mitochondrial redox system.; Authors - Feng Gao
mouse	WB	Elife; Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8.; Authors - Hsiang-Chun Chang ; KD Validated
mouse	WB,IF	FASEB J; Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression.; Authors - Sudhir Kumar ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yaschar (Verified Customer) (06-26-2018)

sample: 20 µg highly purified rat liver mitochondriaVery poor signal intensity even after 30(!) mintues of exposure (see image). Note that this is the best signal I was able to get.Barely any signal in different whole cell lysate.Even after changing the PAGE and Blotting protocols the signal does not get better, incubating over night does not help either.High number of unspecified bands, different lysate and reduction protocols did not help in this regard.I am extremely unsatisfied with this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Isolated rat liver mitochondria (high purity)

ALR Antibody Western Blot, validation (1:1000 dilution) in Isolated rat liver mitochondria (high purity) (Cat no:11293-1-AP)

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