ALKBH5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 67811-1-PBS

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

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Validation Data Gallery

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  WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

  WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67811-1-Ig (same clone as 67811-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67811-1-Ig (same clone as 67811-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67811-1-Ig (same clone as 67811-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67811-1-PBS targets ALKBH5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALKBH5 fusion protein Ag10490
• Full Name: alkB, alkylation repair homolog 5 (E. coli)
• Calculated Molecular Weight: 458 aa, 51 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC062339
• Gene Symbol: ALKBH5
• Gene ID (NCBI): 54890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.