Filter: AllWBIHC

WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)
WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 67811-1-Ig (same clone as 67811-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 67811-1-Ig (same clone as 67811-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 67811-1-Ig (same clone as 67811-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ALKBH5 Monoclonal antibody, PBS Only

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67811-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

67811-1-PBS targets ALKBH5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	ALKBH5 fusion protein Ag10490 Predict reactive species
Full Name	alkB, alkylation repair homolog 5 (E. coli)
Calculated Molecular Weight	458 aa, 51 kDa
Observed Molecular Weight	40-44 kDa
GenBank Accession Number	BC062339
Gene Symbol	ALKBH5
Gene ID (NCBI)	54890
RRID	AB_2918574
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6P6C2
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.