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ALKBH5 Monoclonal antibody

ALKBH5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 67811-1-Ig

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1



Tested Applications

Positive WB detected in4T1 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67811-1-Ig targets ALKBH5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ALKBH5 fusion protein Ag10490
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight40-44 kDa
GenBank Accession NumberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2918574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH5 antibody 67811-1-IgDownload protocol
IHC protocol for ALKBH5 antibody 67811-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Apoptosis

NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer

Authors - Yang Wang
mouseWB

Cell Signal

FTO alleviates cerebral ischemia/reperfusion-induced neuroinflammation by decreasing cGAS mRNA stability in an m6A-dependent manner

Authors - Zhiyong Yu
humanIHC,WB

Sci Rep

ALKBH5 is a prognostic factor and promotes the angiogenesis of glioblastoma

Authors - Yugeng Fan
mouseWB

Acta Physiol (Oxf)

Landscape of transcriptome-wide m6A modification in diabetic liver reveals rewiring of PI3K-Akt signaling after physical exercise

Authors - Cong Chen
humanWB

Nat Commun

M6A reduction relieves FUS-associated ALS granules

Authors - Gaia Di Timoteo